A Protein Factory For High Throughput Structural Biology

Protein Production-UK is the new core facility for recombinant protein production in the Research Complex at Harwell incorporating the technology platforms developed by the OPPF (Oxford Protein Production Facility).

We have a range of highly specialized technologies incorporating robotic systems to enable the high throughput expression, purification of recombinant proteins in E. coli, mammalian and insect cells.

©2010 Medical Research Council

To see the protein production line in action and how we crystallize proteins, please watch the video below

Access

To apply for access, please go to the Instruct website.

 

Recent papers

Salimraj R, Hinchliffe P, Kosmopoulou M, Tyrrell JM, Brem J, van Berkel SS, Verma A, Owens RJ, McDonough MA, Walsh TR, Schofield CJ, Spencer J. Crystal Structures of VIM-1 Complexes Explain Active Site Heterogeneity in VIM-Class Metallo-β-Lactamases. FEBS J. 2018 Nov 15. doi: 10.1111/febs.14695.

Torini JR, Romanello L, Batista FAH, Serrão VHB, Faheem M, Zeraik AE, Bird L, Nettleship J, Reddivari Y, Owens R, DeMarco R, Borges JC, Brandão-Neto J, Pereira HD. The molecular structure of Schistosoma mansoni PNP isoform 2 provides insights into the nucleoside selectivity of PNPs. PLoS One. 2018 Sep 7;13(9):e0203532. doi: 10.1371/journal.pone.0203532.

Yogavel M, Nettleship JE, Sharma A, Harlos K, Jamwal A, Chaturvedi R, Sharma M, Jain V, Chhibber-Goel J, Sharma A. Structure of 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase-dihydropteroate synthase from Plasmodium vivax sheds light on drug resistance. J Biol Chem. 2018 Aug 13. pii: jbc.RA118.00455

Trundová M, Kovaľ T, Owens RJ, Fejfarová K, Dušková J, Kolenko P, Dohnálek J. Highly stable single-strand-specific 3'-nuclease/nucleotidase from Legionella pneumophila. Int J Biol Macromol. 2018 Jul 15;114:776-787.

C. S. Hardman, Y. L. Chen, M. Salimi, R. Jarrett, D. Johnson, V. J. Jarvinen, R. J. Owens, E. Repapi, D. J. Cousins, J. L. Barlow, A. N. J. McKenzie, G. Ogg CD1a presentation of endogenous antigens by group 2 innate lymphoid cells Science Immunology  2017 22 Dec:Vol. 2, Issue 18, eaan5918.

Robinson JI, Baxter EW, Owen RL, Thomsen M, Tomlinson DC, Waterhouse MP, Win SJ, Nettleship JE, Tiede C, Foster RJ, Owens RJ, Fishwick CWG, Harris SA, Goldman A, McPherson MJ, and. Morgan AW Proceedings of the National Academy of Sciences Affimer proteins inhibit immune complex binding to FcγRIIIa with high specificity through competitive and allosteric modes of action Proc.Natl. Acad. Sci USA  2017 ; published ahead of print December 15, 2017, doi:10.1073/pnas.