Oxford Protein Production Facility UK
Registration for the OPPF Practical Course on High Throughput Protein Expression Screening 2015 course is now open.
Click here for more information.
The Oxford Protein Production Facility UK is a structural proteomics facility in the Division of Structural Biology of the Department of Medicine, Oxford University, funded by the Medical Research Council and Biotechnology and Biological Sciences Research Council as a National Resource Centre for protein production and crystallization.
The OPPF-UK aims to promote and facilitate high throughput structural biology for the UK academic community.
The OPPF-UK is located in the Research Complex at Harwell, adjacent to the Diamond Light Source.
©2010 Medical Research Council
We offer parallel vector construction and expression screening and access to high-throughput crystallization using 100nl sitting drops. Please go to the Services tab for details. To apply, please register on our website and then complete a proposal form available under the Services tab. The OPPF-UK is open to all UK academic groups and the use of our facilities is "free at the point of access".
For information about transnational access to this facility, please go to the Instruct website.
Practical and up-to-date, Structural Proteomics: High-Throughput Methods, Second Edition will aid researchers in expanding our knowledge of this vital and expansive area of protein science.
Read more here
Nettleship JE, Watson PJ, Rahman-Huq N, Fairall L, Posner MG, Upadhyay A, Reddivari Y, Chamberlain JM, Kolstoe SE, Bagby S, Schwabe JW, Owens RJ. Transient expression in HEK 293 cells: an alternative to E. coli for the production of secreted and intracellular mammalian proteins. Methods Mol Biol. 2015;1258:209-22.
Hu NJ, Rada H, Rahman N, Nettleship JE, Bird L, Iwata S, Drew D, Cameron AD, Owens RJ. GFP-Based Expression Screening of Membrane Proteins in Insect Cells Using the Baculovirus System. Methods Mol Biol. 2015;1261:197-209.
Bird LE, Rada H, Verma A, Gasper R, Birch J, Jennions M, Lӧwe J, Moraes I, Owens RJ. Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli. J Vis Exp. 2015 Jan 6;(95).
Bird LE, Rada H, Flanagan J, Diprose JM, Gilbert RJ, Owens RJ. Application of In-Fusion™ Cloning for the Parallel Construction of E. coli Expression Vectors. Methods Mol Biol. 2014;1116:209-34.
Tiede C, Tang AA, Deacon SE, Mandal U, Nettleship JE, Owen RL, George SE, Harrison DJ, Owens RJ, Tomlinson DC, McPherson MJ. Adhiron: a stable and versatile peptide display scaffold for molecular recognition applications. Protein Eng Des Sel. 2014 May;27(5):145-55.
Research at the Oxford Protein Production Facility is enabled primarily by grants from the Medical Research Council with additional support from the Biotechnology and Biological Sciences Research Council.
