Protein Production UK

Protein Production UK (PPUK) is a new initiative in the Research Complex that addresses sample preparation for challenging proteins and multicomponent complexes. PPUK will be part of the Rosalind Franklin Institute and closely linked to the Central facilities on the Harwell campus. PPUK aims to develop new and innovative methods and to work in partnership with academic groups and industry to enable uptake of the technology.

PPUK has an established pipeline for protein production based on the technology developed by the Oxford Protein Production Facility (OPPF), involving construction of multi-purpose vectors (e.g. for producing complexes, and membrane proteins), high-throughput expression screening in different cell hosts (microbial, insect and mammalian cells) combined with scale-up to multi-litre culture volumes and downstream processing. New technology platforms that are under development include production of single-domain antibodies for the stabilisation of membrane proteins for analysis by cryo-EM.

©2019 RFI

To see the protein production line in action and how we crystallize proteins, please watch the video below

 

 

 

Recent papers

Tiong Kit Tan, Pramila Rijal, Rolle Rahikainen , Anthony H Keeble , Lisa Schimanski, Saira Hussain , Ruth Harvey, Jack W P Hayes , Jane C Edwards , Rebecca K McLean , Veronica Martini , Miriam Pedrera , Nazia Thakur , Carina Conceicao , Isabelle Dietrich , Holly Shelton , Anna Ludi , Ginette Wilsden , Clare Browning , Adrian K Zagrajek , Dagmara Bialy , Sushant Bhat , Phoebe Stevenson-Leggett , Philippa, Matthew Tully , Katy Moffat , Chris Chiu , Ryan Waters , Ashley Gray , Mehreen Azhar , Valerie Mioulet , Joseph Newman , Amin S Asfor , Alison Burman , Sylvia Crossley , John A Hammond , Elma Tchilian6, Bryan Charleston , Dalan Bailey , Tobias J Tuthill , Simon P Graham , Helen M E Duyvesteyn , Tomas Malinauskas , Jiandong Huo , Julia A Tree 1, Karen R Buttigieg 1, Raymond J Owens , Miles W Carroll , Rodney S Daniels , John W McCauley , David I Stuart , Kuan-Ying A Huang, Mark Howarth , Alain R Townsend  A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV-2 spike protein receptor-binding domain induces potent neutralising antibody responses Nat Commun 2021 Jan 22;12(1):542

Nelson CR, Mrozowich T, Park SM, D'souza S, Henrickson A, Vigar JRJ, Wieden HJ, Owens RJ, Demeler B, Patel TR. Human DDX17 Unwinds Rift Valley Fever Virus Non-Coding RNAs. Int J Mol Sci. 2020 Dec 23;22(1):54.

Angeliki Ditsiou , Chiara Cilibrasi , Nikiana Simigdala , Athanasios Papakyriakou , Leanne Milton-Harris , Viviana Vella , Joanne E. Nettleship, Jae Ho Lo , Shivani Soni , Goar Smbatyan , Panagiota Ntavelou , Teresa Gagliano , Maria Chiara Iachini , Sahir Khurshid, Thomas Simon , Lihong Zhou, Storm Hassell-Hart , Philip Carter, Laurence H. Pearl , Robin L. Owen, Raymond J. Owens, S. Mark Roe, Naomi E. Chayen , Heinz-Josef Lenz , John Spencer , Chrisostomos Prodromou , Apostolos Klinakis , Justin Stebbing, Georgios Giamas The structure-function relationship of oncogenic LMTK3 Sci. Adv. 2020; 6 : eabc3099 13 November 2020

Solomon-Degefa H, Gebauer JM, Jeffries CM, Freiburg CD, Meckelburg P, Bird LE, Baumann U, Svergun DI, Owens RJ, Werner JM, Behrmann E, Paulsson M, Wagener R. Structure of a collagen VI α3 chain VWA domain array: adaptability and functional implications of myopathy causing mutations. J Biol Chem. 2020 Jul 21:jbc.RA120.014865. doi: 10.1074/jbc.RA120.014865. Online ahead of print.

Graham SP, McLean RK, Spencer AJ, Belij-Rammerstorfer S, Wright D, Ulaszewska M, Edwards JC, Hayes JWP, Martini V, Thakur N, Conceicao C, Dietrich I, Shelton H, Waters R, Ludi A, Wilsden G, Browning C, Bialy D, Bhat S, Stevenson-Leggett P, Hollinghurst P, Gilbride C, Pulido D, Moffat K, Sharpe H, Allen E, Mioulet V, Chiu C, Newman J, Asfor AS, Burman A, Crossley S, Huo J, Owens RJ, Carroll M, Hammond JA, Tchilian E, Bailey D, Charleston B, Gilbert SC, Tuthill TJ, Lambe T.  Evaluation of the immunogenicity of prime-boost vaccination with the replication-deficient viral vectored COVID-19 vaccine candidate ChAdOx1 nCoV-19.NPJ Vaccines. 2020 Jul 27;5:69. doi: 10.1038/s41541-020-00221-3. eCollection 2020.

Zhou D, Duyvesteyn HME, Chen CP, Huang CG, Chen TH, Shih SR, Lin YC, Cheng CY, Cheng SH, Huang YC, Lin TY, Ma C, Huo J, Carrique L, Malinauskas T, Ruza RR, Shah PNM, Tan TK, Rijal P, Donat RF, Godwin K, Buttigieg KR, Tree JA, Radecke J, Paterson NG, Supasa P, Mongkolsapaya J, Screaton GR, Carroll MW, Gilbert-Jaramillo J, Knight ML, James W, Owens RJ, Naismith JH, Townsend AR, Fry EE, Zhao Y, Ren J, Stuart DI, Huang KA. Structural basis for the neutralization of SARS-CoV-2 by an antibody from a convalescent patient. Nat Struct Mol Biol. 2020 Jul 31. doi: 10.1038/s41594-020-0480-y. Online ahead of print.

Huo J, Zhao Y, Ren J, Zhou D, Duyvesteyn HME, Ginn HM, Carrique L, Malinauskas T, Ruza RR, Shah PNM, Tan TK, Rijal P, Coombes N, Bewley KR, Tree JA, Radecke J, Paterson NG, Supasa P, Mongkolsapaya J, Screaton GR, Carroll M, Townsend A, Fry EE, Owens RJ, Stuart DI. Neutralization of SARS-CoV-2 by Destruction of the Prefusion Spike. Cell Host Microbe. 2020 Jun 19:S1931-3128(20)30351-6.

Huo J, Le Bas A, Ruza RR, Duyvesteyn HME, Mikolajek H, Malinauskas T, Tan TK, Rijal P, Dumoux M, Ward PN, Ren J, Zhou D, Harrison PJ, Weckener M, Clare DK, Vogirala VK, Radecke J, Moynié L, Zhao Y, Gilbert-Jaramillo J, Knight ML, Tree JA, Buttigieg KR, Coombes N, Elmore MJ, Carroll MW, Carrique L, Shah PNM, James W, Townsend AR, Stuart DI, Owens RJ, Naismith JH. Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2. Nat Struct Mol Biol. 2020 Jul 13. doi: 10.1038/s41594-020-0469-6

Kim YC, Dumoux M, Owens RJ, Reyes-Sandoval A. Optimization of Small-Scale Production of Zika Virus Envelope Glycoprotein by Transient Expression in HEK293 Cells for ELISA. Methods Mol Biol. 2020;2142:103-112.

 

 

 

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