A Protein Factory For High Throughput Structural Biology

Protein Production-UK is the new core facility for recombinant protein production in the Research Complex at Harwell incorporating the technology platforms developed by the OPPF (Oxford Protein Production Facility).

We have a range of highly specialized technologies incorporating robotic systems to enable the high throughput expression, purification of recombinant proteins in E. coli, mammalian and insect cells.

©2010 Medical Research Council

To see the protein production line in action and how we crystallize proteins, please watch the video below

Access

To apply for access, please go to the Instruct website.

 

Recent papers

Yogavel M, Nettleship JE, Sharma A, Harlos K, Jamwal A, Chaturvedi R, Sharma M, Jain V, Chhibber-Goel J, Sharma A. Structure of 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase-dihydropteroate synthase from Plasmodium vivax sheds light on drug resistance. J Biol Chem. 2018 Aug 13. pii: jbc.RA118.00455

Trundová M, Kovaľ T, Owens RJ, Fejfarová K, Dušková J, Kolenko P, Dohnálek J. Highly stable single-strand-specific 3'-nuclease/nucleotidase from Legionella pneumophila. Int J Biol Macromol. 2018 Jul 15;114:776-787.

C. S. Hardman, Y. L. Chen, M. Salimi, R. Jarrett, D. Johnson, V. J. Jarvinen, R. J. Owens, E. Repapi, D. J. Cousins, J. L. Barlow, A. N. J. McKenzie, G. Ogg CD1a presentation of endogenous antigens by group 2 innate lymphoid cells Science Immunology  2017 22 Dec:Vol. 2, Issue 18, eaan5918.

Robinson JI, Baxter EW, Owen RL, Thomsen M, Tomlinson DC, Waterhouse MP, Win SJ, Nettleship JE, Tiede C, Foster RJ, Owens RJ, Fishwick CWG, Harris SA, Goldman A, McPherson MJ, and. Morgan AW Proceedings of the National Academy of Sciences Affimer proteins inhibit immune complex binding to FcγRIIIa with high specificity through competitive and allosteric modes of action Proc.Natl. Acad. Sci USA  2017 ; published ahead of print December 15, 2017, doi:10.1073/pnas.

Mina JG, Thye JK, Alqaisi AQI, Bird LE, Dods RH, Grøftehauge MK, Mosely JA, Pratt S, Shams-Eldin H, Schwarz RT, Pohl E, Denny PW. Functional and phylogenetic evidence of a bacterial origin for the first enzyme in sphingolipid biosynthesis in a phylum of eukaryotic protozoan parasites.J Biol Chem. 2017 Jul 21;292(29):12208-12219.

Romanello L, Serrão VHB, Torini JR, Bird LE, Nettleship JE, Rada H, Reddivari Y, Owens RJ, DeMarco R, Brandão-Neto J, Pereira HD. Structural and kinetic analysis of Schistosoma mansoni Adenylosuccinate Lyase (SmADSL). Mol Biochem Parasitol. 2017 Jun;214:27-35

Tiede C, Bedford R, Heseltine SJ, Smith G, Wijetunga I, Ross R, AlQallaf D, Roberts AP, Balls A, Curd A, Hughes RE, Martin H, Needham SR, Zanetti-Domingues LC, Sadigh Y, Peacock TP, Tang AA, Gibson N, Kyle H, Platt GW, Ingram N, Taylor T, Coletta LP, Manfield I, Knowles M, Bell S, Esteves F, Maqbool A, Prasad RK, Drinkhill M, Bon RS, Patel V, Goodchild SA, Martin-Fernandez M, Owens RJ, Nettleship JE, Webb ME, Harrison M, Lippiat JD, Ponnambalam S, Peckham M, Smith A, Ferrigno PK, Johnson M, McPherson MJ, Tomlinson DC. Affimer proteins are versatile and renewable affinity reagents. Elife. 2017 Jun 27;6. pii: e24903.