Oxford Protein Production Facility UK
The Oxford Protein Production Facility-UK (OPPF-UK) is a core facility for protein production and crystallization at the Research Complex at Harwell.
We have developed a range of highly specialized technologies incorporating robotic systems to enable the high throughput expression, purification and crystallization of recombinant proteins.
The OPPF-UK is located in the Research Complex at Harwell, adjacent to the Diamond Light Source.
©2010 Medical Research Council
To see the OPPF-UK in action, watch the video below
To apply for access to the OPPF baculovirus construction and expression screening platform, please go to the Instruct website.
Recent book edited by Ray Owens titled Structural Proteomics : High-Throughput Methods is available now.
Faheem M, Martins-de-Sa D, Vidal JF, Álvares AC, Brandão-Neto J, Bird LE, Tully MD, von Delft F, Souto BM, Quirino BF, Freitas SM, Barbosa JA. Functional and structural characterization of a novel putative cysteine protease cell wall-modifying multi-domain enzyme selected from a microbial metagenome. Sci Rep. 2016 Dec 9;6:38031
Valente M, Timm J, Castillo-Acosta VM, Ruiz-Pérez LM, Balzarini T, Nettleship JE, Bird LE, Rada H, Wilson KS, González-Pacanowska D. Cell cycle regulation and novel structural features of thymidine kinase, an essential enzyme in Trypanosoma brucei. Mol Microbiol. 2016 Jul 18. doi: 10.1111/mmi.13467. [Epub ahead of print]
Bird LE, Nettleship JE, Järvinen V, Rada H, Verma A, Owens RJ. Expression Screening of Integral Membrane Proteins by Fusion to Fluorescent Reporters. Adv Exp Med Biol. 2016;922:1-11. doi: 10.1007/978-3-319-35072-1_1.
Bayley CP, Ruiz Nivia HD, Dajani R, Jowitt TA, Collins RF, Rada H, Bird LE, Baldock C.Diversity between mammalian tolloid proteinases: Oligomerisation and non-catalytic domains influence activity and specificity. Sci Rep. 2016 Feb 23;6:21456. doi: 10.1038/srep21456
Roatsch M, Robaa D, Pippel M, Nettleship JE, Reddivari Y, Bird LE, Hoffmann I, Franz H, Owens RJ, Schüle R, Flaig R, Sippl W, Jung M.Substituted 2-(2-aminopyrimidin-4-yl)pyridine-4-carboxylates as potent inhibitors of JumonjiC domain-containing histone demethylases. Future Med Chem. 2016 Mar 14.
Research at the Oxford Protein Production Facility is enabled primarily by grants from the Medical Research Council with additional support from the Biotechnology and Biological Sciences Research Council.
