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A Protein Factory For High Throughput Structural Biology

Protein Production UK (PPUK) is a new initiative in the Research Complex that addresses sample preparation for challenging proteins and multicomponent complexes. PPUK will be part of the Rosalind Franklin Institute and closely linked to the Central facilities on the Harwell campus. PPUK aims to develop new and innovative methods and to work in partnership with academic groups and industry to enable uptake of the technology.

PPUK has an established pipeline for protein production based on the technology developed by the Oxford Protein Production Facility, involving construction of multi-purpose vectors (e.g. for producing complexes, and membrane proteins), high-throughput expression screening in different cell hosts (microbial, insect and mammalian cells) combined with scale-up to multi-litre culture volumes and downstream processing. New technology platforms that are under development include production of single-domain antibodies for the stabilisation of membrane proteins for analysis by cryo-EM.

OPPF-UK lab
©2010 Medical Research Council

To see the protein production line in action and how we crystallize proteins, please watch the video below

 

 

 

The Oxford Protein Production Facility was established primarily by grants from the Medical Research Council with additional support from the Biotechnology and Biological Sciences Research Council .

Medical Research Council

Biotechnology and Biological Sciences Research Council