Oxford Protein Production Facility UK
Protein Production-UK is the new core facility for recombinant protein production in the Research Complex at Harwell incorporating the technology platforms developed by the OPPF (Oxford Protein Production Facility).
We have a range of highly specialized technologies incorporating robotic systems to enable the high throughput expression, purification of recombinant proteins in E. coli, mammalian and insect cells.
©2010 Medical Research Council
To see the protein production line in action and how we crystallize proteins, please watch the video below
To apply for access, please go to the Instruct website.
C. S. Hardman, Y. L. Chen, M. Salimi, R. Jarrett, D. Johnson, V. J. Jarvinen, R. J. Owens, E. Repapi, D. J. Cousins, J. L. Barlow, A. N. J. McKenzie, G. Ogg CD1a presentation of endogenous antigens by group 2 innate lymphoid cells Science Immunology 2017 22 Dec:Vol. 2, Issue 18, eaan5918.
Robinson JI, Baxter EW, Owen RL, Thomsen M, Tomlinson DC, Waterhouse MP, Win SJ, Nettleship JE, Tiede C, Foster RJ, Owens RJ, Fishwick CWG, Harris SA, Goldman A, McPherson MJ, and. Morgan AW Proceedings of the National Academy of Sciences Affimer proteins inhibit immune complex binding to FcγRIIIa with high specificity through competitive and allosteric modes of action Proc.Natl. Acad. Sci USA 2017 ; published ahead of print December 15, 2017, doi:10.1073/pnas.
Mina JG, Thye JK, Alqaisi AQI, Bird LE, Dods RH, Grøftehauge MK, Mosely JA, Pratt S, Shams-Eldin H, Schwarz RT, Pohl E, Denny PW. Functional and phylogenetic evidence of a bacterial origin for the first enzyme in sphingolipid biosynthesis in a phylum of eukaryotic protozoan parasites.J Biol Chem. 2017 Jul 21;292(29):12208-12219.
Morra R, Young K, Casas-Mao D, Dixon N, Bird LE. Optimization of Membrane Protein Production Using Titratable Strains of E. coli. Methods Mol Biol. 2017;1586:83-107.
Romanello L, Serrão VHB, Torini JR, Bird LE, Nettleship JE, Rada H, Reddivari Y, Owens RJ, DeMarco R, Brandão-Neto J, Pereira HD. Structural and kinetic analysis of Schistosoma mansoni Adenylosuccinate Lyase (SmADSL). Mol Biochem Parasitol. 2017 Jun;214:27-35
Tiede C, Bedford R, Heseltine SJ, Smith G, Wijetunga I, Ross R, AlQallaf D, Roberts AP, Balls A, Curd A, Hughes RE, Martin H, Needham SR, Zanetti-Domingues LC, Sadigh Y, Peacock TP, Tang AA, Gibson N, Kyle H, Platt GW, Ingram N, Taylor T, Coletta LP, Manfield I, Knowles M, Bell S, Esteves F, Maqbool A, Prasad RK, Drinkhill M, Bon RS, Patel V, Goodchild SA, Martin-Fernandez M, Owens RJ, Nettleship JE, Webb ME, Harrison M, Lippiat JD, Ponnambalam S, Peckham M, Smith A, Ferrigno PK, Johnson M, McPherson MJ, Tomlinson DC. Affimer proteins are versatile and renewable affinity reagents. Elife. 2017 Jun 27;6. pii: e24903.
West NR, Hegazy AN, Owens BMJ, Bullers SJ, Linggi B, Buonocore S, Coccia M, Görtz D, This S, Stockenhuber K, Pott J, Friedrich M, Ryzhakov G, Baribaud F, Brodmerkel C, Cieluch C, Rahman N, Müller-Newen G, Owens RJ, Kühl AA, Maloy KJ, Plevy SE; Oxford IBD Cohort Investigators, Keshav S, Travis SPL, Powrie F. Oncostatin M drives intestinal inflammation and predicts response to tumor necrosis factor-neutralizing therapy in patients with inflammatory bowel disease. Nat Med. 2017 May;23(5):579-589
The Oxford Protein Production Facility was established primarily by grants from the Medical Research Council with additional support from the Biotechnology and Biological Sciences Research Council .
