Oxford Protein Production Facility UK
The Oxford Protein Production Facility UK is a structural proteomics facility in the Division of Structural Biology of the Department of Medicine, Oxford University, funded by the Medical Research Council and Biotechnology and Biological Sciences Research Council as a National Resource Centre for protein production and crystallization.
The OPPF-UK aims to promote and facilitate high throughput structural biology for the UK academic community. It is the first stage in a structural proteomics programme for the UK and represents an essential stepping stone toward the practical exploitation of the wealth of information coming from the human genome sequencing projects.
The OPPF-UK is located in the Research Complex at Harwell, adjacent to the Diamond Light Source.
©2010 Medical Research Council
We offer parallel vector construction and expression screening and access to high-throughput crystallization using 100nl sitting drops. Please go to the Services tab for details. To apply, please register on our website and then complete a proposal form available under the Services tab. The OPPF-UK is open to all UK academic groups and the use of our facilities is "free at the point of access".
For information about transnational access to this facility, please go to the Instruct website.
Bird LE, Rada H, Flanagan J, Diprose JM, Gilbert RJ, Owens RJ. Application of In-Fusion™ Cloning for the Parallel Construction of E. coli Expression Vectors. Methods Mol Biol. 2014;1116:209-34.
Knox R, Nettleship JE, Chang VT, Hui ZK, Santos AM, Rahman N, Ho LP, Owens RJ, Davis SJ.A streamlined implementation of the glutamine synthetase-based protein expression system.BMC Biotechnol. 2013 Sep 24;13(1):74.
van Berkel SS, Nettleship JE, Leung IK, Brem J, Choi H, Stuart DI, Claridge, TD, McDonough MA, Owens RJ, Ren J, Schofield CJ. Binding of (5S)-Penicilloic Acid to Penicillin Binding Protein 3. ACS Chem Biol. 2013 Aug. 15.
van Berkel SS, Brem J, Rydzik AM, Salimraj R, Cain R, Verma A, Owens RJ, Fishwick CW, Spencer J, Schofield CJ. An Assay Platform for Clinically Relevant Metallo-β-Lactamases. J Med Chem. 2013 Sep 12;56(17):6945-53.
Nettleship JE, Ren J, Scott DJ, Rahman N, Hatherley D, Zhao Y, Stuart DI, Barclay AN, Owens RJ. Crystal structure of signal regulatory protein gamma (SIRPgamma) in complex with an antibody Fab fragment. BMC Struct Biol. 2013 Jul 4;13(1):13.
Aricescu AR, Owens RJ. Expression of recombinant glycoproteins in mammalian cells: towards an integrative approach to structural biology. Curr Opin Struct Biol. 2013 Jun;23(3):345-56.
Research at the Oxford Protein Production Facility is enabled primarily by grants from the Medical Research Council with additional support from the Biotechnology and Biological Sciences Research Council.
