Araujo-Montoya BO, Senger MR, Gomes BF, Harris G, Owens RJ, Silva-Jr FP. Schistosoma mansoni cathepsin D1: Biochemical and biophysical characterization of the recombinant enzyme expressed in HEK293T cells. Protein Expr Purif. 2019 Nov 8:105532.

Nettleship JE, Rada H, Owens RJ. Overview of a High-Throughput Pipeline for Streamlining the Production of Recombinant Proteins. Methods Mol Biol. 2019;2025:33-49.

Torini JR, de Freitas Fernandes A, Balasco Serrão VH, Romanello L, Bird LE, Nettleship JE, Owens RJ, Brandão-Neto J, Zeraik AE, DeMarco R, D'Muniz Pereira H. Characterization of a Schistosoma mansoni NDPK expressed in sexual and digestive organs. Mol Biochem Parasitol. 2019 May 16:111187. doi: 10.1016/j.

Ren J, Nettleship JE, Harris G, Mwangi W, Rhaman N, Grant C, Kotecha A, Fry E, Charleston B, Stuart DI, Hammond J, Owens RJ.The role of the light chain in the structure and binding activity of two cattle antibodies that neutralize bovine respiratory syncytial virus. Mol Immunol. 2019 May 14;112:123-130.

Awanye AM, Chang CM, Wheeler JX, Chan H, Marsay L, Dold C, Rollier CS, Bird LE, Nettleship JE, Owens RJ, Pollard AJ, Derrick JP. Immunogenicity profiling of protein antigens from capsular group B Neisseria meningitidis. Sci Rep. 2019 May 2;9(1):6843.

Ahmed AR, Owens RJ,  Stubbs CD, Parker AW, Hitchman R, Yadav RB, Dumoux M, Hawes C,  & Botchway SW. Direct imaging of the recruitmentand phosphorylation of S6K1 in the mTORC1 pathway in living cells Scientific Reports  2019) 9:3408

Romanello L, Zeraik AE, de Freitas Fernandes A, Torini JR, Bird LE, Nettleship JE, Rada H, Reddivari Y, Owens RJ, Serrão VHB, DeMarco R, Brandão-Neto J, Pereira HD. In vitro and in vivo characterization of the multiple isoforms of Schistosoma mansoni Hypoxanthine-guanine phosphoribosyltransferases. Mol Biochem Parasitol. 2019 Feb 14. pii: S0166-6851(18)30243-3. doi: 10.1016/j.molbiopara.2019.02.005.

Sabaratnam K, Renner M, Paesen G, Harlos K, Nair V, Owens RJ, Grimes JM. Insights from the crystal structure of the chicken CREB3 bZIP suggest members of the CREB3 subfamily transcription factors may be activated in response to oxidative stress. Protein Sci. 2019 Jan 17. doi: 10.1002/pro.3573


Kim YC, Lopez-Camacho C, Nettleship JE, Rahman N, Hill ML, Silva-Reyes L, Ortiz-Martinez G, Figueroa-Aguilar G, Mar MA, Vivanco-Cid H, Rollier CS, Zitzmann N, Viveros-Sandoval ME, Owens RJ, Reyes-Sandoval A. Optimization of Zika virus envelope protein production for ELISA and correlation of antibody titers with virus neutralization in Mexican patients from an arbovirus endemic region. Virol J. 2018 Dec 27;15(1):193.

Salimraj R, Hinchliffe P, Kosmopoulou M, Tyrrell JM, Brem J, van Berkel SS, Verma A, Owens RJ, McDonough MA, Walsh TR, Schofield CJ, Spencer J. Crystal Structures of VIM-1 Complexes Explain Active Site Heterogeneity in VIM-Class Metallo-β-Lactamases. FEBS J. 2018 Nov 15. doi: 10.1111/febs.14695.

Torini JR, Romanello L, Batista FAH, Serrão VHB, Faheem M, Zeraik AE, Bird L, Nettleship J, Reddivari Y, Owens R, DeMarco R, Borges JC, Brandão-Neto J, Pereira HD. The molecular structure of Schistosoma mansoni PNP isoform 2 provides insights into the nucleoside selectivity of PNPs. PLoS One. 2018 Sep 7;13(9):e0203532. doi: 10.1371/journal.pone.0203532.

Shimmon G, Kotecha A, Ren J, Asfor AS, Newman J, Berryman S, Cottam EM, Gold S, Tuthill TJ, King DP, Brocchi E, King AMQ, Owens R, Fry EE, Stuart DI, Burman A, Jackson T Generation and characterisation of recombinant FMDV antibodies: Applications for advancing diagnostic and laboratory assays. PLoS One. 2018 Aug 16;13(8):e0201853.

Yogavel M, Nettleship JE, Sharma A, Harlos K, Jamwal A, Chaturvedi R, Sharma M, Jain V, Chhibber-Goel J, Sharma A. Structure of 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase-dihydropteroate synthase from Plasmodium vivax sheds light on drug resistance. J Biol Chem. 2018 Aug 13. pii: jbc.RA118.00455

Hannon C, Cruz-Migoni A, Platonova O1, Owen RL, Nettleship JE, Miller A, Carr SB, Harris G, Rabbitts TH, Phillips SEV. Cloning, purification and structure determination of the HIV integrase-binding domain of lens epithelium-derived growth factor. Acta Crystallogr F Struct Biol Commun. 2018 Mar 1;74(Pt 3):143-149.

Trundová M, Kovaľ T, Owens RJ, Fejfarová K, Dušková J, Kolenko P, Dohnálek J. Highly stable single-strand-specific 3'-nuclease/nucleotidase from Legionella pneumophila. Int J Biol Macromol. 2018 Jul 15;114:776-787.


C. S. Hardman, Y. L. Chen, M. Salimi, R. Jarrett, D. Johnson, V. J. Jarvinen, R. J. Owens, E. Repapi, D. J. Cousins, J. L. Barlow, A. N. J. McKenzie, G. Ogg CD1a presentation of endogenous antigens by group 2 innate lymphoid cells Science Immunology  2017 22 Dec:Vol. 2, Issue 18, eaan5918.

Robinson JI, Baxter EW, Owen RL, Thomsen M, Tomlinson DC, Waterhouse MP, Win SJ, Nettleship JE, Tiede C, Foster RJ, Owens RJ, Fishwick CWG, Harris SA, Goldman A, McPherson MJ, and. Morgan AW Proceedings of the National Academy of Sciences Affimer proteins inhibit immune complex binding to FcγRIIIa with high specificity through competitive and allosteric modes of action Proc.Natl. Acad. Sci USA  2017 ; published ahead of print December 15, 2017, doi:10.1073/pnas.

Mina JG, Thye JK, Alqaisi AQI, Bird LE, Dods RH, Grøftehauge MK, Mosely JA, Pratt S, Shams-Eldin H, Schwarz RT, Pohl E, Denny PW. Functional and phylogenetic evidence of a bacterial origin for the first enzyme in sphingolipid biosynthesis in a phylum of eukaryotic protozoan parasites.J Biol Chem. 2017 Jul 21;292(29):12208-12219.

Morra R, Young K, Casas-Mao D, Dixon N, Bird LE. Optimization of Membrane Protein Production Using Titratable Strains of E. coli. Methods Mol Biol. 2017;1586:83-107.

Romanello L, Serrão VHB, Torini JR, Bird LE, Nettleship JE, Rada H, Reddivari Y, Owens RJ, DeMarco R, Brandão-Neto J, Pereira HD. Structural and kinetic analysis of Schistosoma mansoni Adenylosuccinate Lyase (SmADSL). Mol Biochem Parasitol. 2017 Jun;214:27-35

Tiede C, Bedford R, Heseltine SJ, Smith G, Wijetunga I, Ross R, AlQallaf D, Roberts AP, Balls A, Curd A, Hughes RE, Martin H, Needham SR, Zanetti-Domingues LC, Sadigh Y, Peacock TP, Tang AA, Gibson N, Kyle H, Platt GW, Ingram N, Taylor T, Coletta LP, Manfield I, Knowles M, Bell S, Esteves F, Maqbool A, Prasad RK, Drinkhill M, Bon RS, Patel V, Goodchild SA, Martin-Fernandez M, Owens RJ, Nettleship JE, Webb ME, Harrison M, Lippiat JD, Ponnambalam S, Peckham M, Smith A, Ferrigno PK, Johnson M, McPherson MJ, Tomlinson DC. Affimer proteins are versatile and renewable affinity reagents. Elife. 2017 Jun 27;6. pii: e24903.

West NR, Hegazy AN, Owens BMJ, Bullers SJ, Linggi B, Buonocore S, Coccia M, Görtz D, This S, Stockenhuber K, Pott J, Friedrich M, Ryzhakov G, Baribaud F, Brodmerkel C, Cieluch C, Rahman N, Müller-Newen G, Owens RJ, Kühl AA, Maloy KJ, Plevy SE; Oxford IBD Cohort Investigators, Keshav S, Travis SPL, Powrie F. Oncostatin M drives intestinal inflammation and predicts response to tumor necrosis factor-neutralizing therapy in patients with inflammatory bowel disease. Nat Med. 2017 May;23(5):579-589


Faheem M, Martins-de-Sa D, Vidal JF, Álvares AC, Brandão-Neto J, Bird LE, Tully MD, von Delft F, Souto BM, Quirino BF, Freitas SM, Barbosa JA. Functional and structural characterization of a novel putative cysteine protease cell wall-modifying multi-domain enzyme selected from a microbial metagenome. Sci Rep. 2016 Dec 9;6:38031

Mas-Y-Mas S, Barbon M, Teyssier C, Déméné H, Carvalho JE, Bird LE, Lebedev A, Fattori J, Schubert M, Dumas C, Bourguet W, le Maire A. The Human Mixed Lineage Leukemia 5 (MLL5), a Sequentially and Structurally Divergent SET Domain-Containing Protein with No Intrinsic Catalytic Activity. PLoS One. 2016 Nov 3;11(11):e0165139.

Palazzo, L., Daniels, C.M., Nettleship, J.E., Rahman, N., McPherson, R.L., Ong, S.E., Kato, K., Nureki, O., Leung, A.K., Ahel, I., (2016) " ENPP1 processes protein ADP-ribosylation in vitro". FEBS J.2016 Sep;283(18):3371-88

Asiani, K. R., Williams, H., Bird, L., Jenner, M., Searle, M. S., Hobman., J. L., Scott, D. J. and Soultanas, P. (2016), "SilE is an intrinsically disordered periplasmic “molecular sponge” involved in bacterial silver resistance". Molecular Microbiology. Sep;101(5):731-42

Lanyon-Hogg, T., Masumoto, N., Bodakh, G., Konitsiotis, A.D., Thinon, E., Rodgers, U.R., Owens, R.J., Magee, A.I., Tate, E.W., (2016) "Synthesis and characterisation of 5-acyl-6,7-dihydrothieno[3,2-c]pyridine inhibitors of Hedgehog acyltransferase". Data Brief. 10;7:257-81.

Valente M, Timm J, Castillo-Acosta VM, Ruiz-Pérez LM, Balzarini T, Nettleship JE, Bird LE, Rada H, Wilson KS, González-Pacanowska D. Cell cycle regulation and novel structural features of thymidine kinase, an essential enzyme in Trypanosoma brucei. Mol Microbiol. 2016 Jul 18. doi: 10.1111/mmi.13467. [Epub ahead of print]

Bird LE, Nettleship JE, Järvinen V, Rada H, Verma A, Owens RJ. Expression Screening of Integral Membrane Proteins by Fusion to Fluorescent Reporters. Adv Exp Med Biol. 2016;922:1-11. doi: 10.1007/978-3-319-35072-1_1.

Bayley CP, Ruiz Nivia HD, Dajani R, Jowitt TA, Collins RF, Rada H, Bird LE, Baldock C.Diversity between mammalian tolloid proteinases: Oligomerisation and non-catalytic domains influence activity and specificity. Sci Rep. 2016 Feb 23;6:21456. doi: 10.1038/srep21456.PMID: 26902455

Roatsch M, Robaa D, Pippel M, Nettleship JE, Reddivari Y, Bird LE, Hoffmann I, Franz H, Owens RJ, Schüle R, Flaig R, Sippl W, Jung M.Substituted 2-(2-aminopyrimidin-4-yl)pyridine-4-carboxylates as potent inhibitors of JumonjiC domain-containing histone demethylases. Future Med Chem. 2016 Mar 14. [Epub ahead of print] PMID: 26971619.

Ren J, Nettleship JE, Males A, Stuart DI, Owens RJ. Crystal structures of penicillin-binding protein 3 in complexes with azlocillin and cefoperazone in both acylated and deacylated forms. FEBS Lett. 2016 Jan;590(2):288-97. doi: 10.1002/1873-3468.12054. Epub 2016 Jan 23.


Arena de Souza V, Scott DJ, Nettleship JE, Rahman N, Charlton MH, Walsh MA, Owens RJ. Comparison of the Structure and Activity of Glycosylated and Aglycosylated Human Carboxylesterase 1. PLoS One. 2015 Dec 11;10(12):e0143919. doi: 10.1371/journal.pone.0143919. eCollection 2015.

Lukacik P, Lobley CM, Bumann M, Arena de Souza V, Owens RJ, O'Toole PW, Walsh MA. High-resolution structures of Lactobacillus salivarius transketolase in the presence and absence of thiamine pyrophosphate.Acta Crystallogr F Struct Biol Commun. 2015 Oct 1;71(Pt 10):1327-34.

Liu C, Zhao Y, He W, Wang W, Chen Y, Zhang S, Ma Y, Gohda J, Ishida T, Walter TS, Owens RJ, Stuart DI, Ren J, Gao B. A RANKL mutant used as an inter-species vaccine for efficient immunotherapy of osteoporosis. Sci Rep. 2015 Sep 28;5:14150.

Lanyon-Hogg T, Masumoto N, Bodakh G, Konitsiotis AD, Thinon E, Rodgers UR, Owens RJ, Magee AI, Tate EW. Click chemistry armed enzyme-linked immunosorbent assay to measure palmitoylation by hedgehog acyltransferase. Anal Biochem. 2015 Sep 1;490:66-72.

Timm J, Bosch-Navarrete C, Recio E, Nettleship JE, Rada H, Gonzalez-Pacanowska, Wilson KE. Structural and Kinetic Characterization of Thymidine Kinase from Leishmania major. PLoS Neglected Tropical Diseases. 2015;9(5):e0003781.

Beale JH, Parker JL, Samsudin F, Barrett AL, Senan A, Bird LE, Scott D, Owens RJ, Sansom MS, Tucker SJ, Meredith D, Fowler PW, Newstead S. Crystal Structures of the Extracellular Domain from PepT1 and PepT2 Provide Novel Insights into Mammalian Peptide Transport. Structure. 2015  Oct 6;23(10):1889-99.

Srivastava M, Duan G, Kershaw NJ, Athanasopoulos V, Yeo JH, Ose T, Hu D, Brow SH, Jergic S, Patel HR, Pratama A, Richards S, Verma A, Jones EY, Heissmeyer V,Preiss T, Dixon NE, Chong MM, Babon JJ, Vinuesa CG. Roquin binds microRNA-146a and Argonaute2 to regulate microRNA homeostasis. Nat Commun. 2015 Feb 20;6:6253.

Nettleship JE, Watson PJ, Rahman-Huq N, Fairall L, Posner MG, Upadhyay A, Reddivari Y, Chamberlain JM, Kolstoe SE, Bagby S, Schwabe JW, Owens RJ. Transient expression in HEK 293 cells: an alternative to E. coli for the production of secreted and intracellular mammalian proteins. Methods Mol Biol. 2015;1258:209-22. doi: 10.1007/978-1-4939-2205-5_11. Review.

Bird LE, Rada H, Verma A, Gasper R, Birch J, Jennions M, Lӧwe J, Moraes I, Owens RJ. Green fluorescent protein-based expression screening of membrane proteins in Escherichia coli. J Vis Exp. 2015 Jan 6;(95):e52357. doi: 10.3791/52357.

Hu, N,J., Rada, H., Rahman, N., Nettleship, J.E., Bird, L., Iwata, S., Drew, D., Cameron, A.D., Owens, R.J. (2015) GFP-Based Expression Screening of Membrane Proteins in Insect Cells Using the Baculovirus System Methods in Molecular Biology - Structural Proteomics 1261, 197-209.


Itkonen JM, Urtti A, Bird LE, Sarkhel S. Codon optimization and factorial screening for enhanced soluble expression of human ciliary neurotrophic factor in Escherichia coli. BMC Biotechnol. 2014 Nov 14;14:92. doi: 10.1186/s12896-014-0092-x.

Vieira D, Figueiredo TA, Verma A, Sobral RG, Ludovice AM, de Lencastre H, Trincao J. Purification, crystallization and preliminary X-ray diffraction analysis of GatD, a glutamine amidotransferase-like protein from Staphylococcus aureus peptidoglycan. Acta Crystallogr F Struct Biol Commun. 2014 May;70(Pt 5):632-5. doi: 10.1107/S2053230X14007298. Epub 2014 Apr 17.

Tiede C, Tang AA, Deacon SE, Mandal U, Nettleship JE, Owen RL, George SE, Harrison DJ, Owens RJ, Tomlinson DC, McPherson MJ. Adhiron: a stable and versatile peptide display scaffold for molecular recognition applications. Protein Eng Des Sel. 2014 May;27(5):145-55. doi: 10.1093/protein/gzu007. Epub 2014 Mar 25.

Bird LE, Rada H, Flanagan J, Diprose JM, Gilbert RJ, Owens RJ. Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors. Methods Mol Biol. 2014;1116:209-34. doi: 10.1007/978-1-62703-764-8_15.


Makena A, van Berkel SS, Lejeune C, Owens RJ, Verma A, Salimraj R, Spencer J, Brem J, Schofield CJ. Chromophore-linked substrate (CLS405): probing metallo-β-lactamase activity and inhibition. ChemMedChem. 2013 Dec;8(12):1923-9. doi: 10.1002/cmdc.201300350. Epub 2013 Oct 25.

Knox R, Nettleship JE, Chang VT, Hui ZK, Santos AM, Rahman N, Ho LP, Owens RJ, Davis SJ. A streamlined implementation of the glutamine synthetase-based protein expression system. BMC Biotechnol. 2013 Sep 24;13:74. doi: 10.1186/1472-6750-13-74.

van Berkel SS, Nettleship JE, Leung IK, Brem J, Choi H, Stuart DI, Claridge TD, McDonough MA, Owens RJ, Ren J, Schofield CJ. Binding of (5S)-penicilloic acid to penicillin binding protein 3. ACS Chem Biol. 2013 Oct 18;8(10):2112-6. doi: 10.1021/cb400200h. Epub 2013 Aug 15.

van Berkel SS, Brem J, Rydzik AM, Salimraj R, Cain R, Verma A, Owens RJ, Fishwick CW, Spencer J, Schofield CJ. (2013) An Assay Platform for Clinically Relevant Metallo-β-Lactamases J Med Chem. 56, 6945-6953.

Nettleship JE, Ren J, Scott DJ, Rahman N, Hatherley D, Zhao Y, Stuart DI, Barclay AN, Owens RJ. Crystal structure of signal regulatory protein gamma (SIRPγ) in complex with an antibody Fab fragment. BMC Struct Biol. 2013 Jul 4;13:13. doi: 10.1186/1472-6807-13-13. PMID: 23826770

Byrne RT, Whelan F, Aller P, Bird LE, Dowle A, Lobley CM, Reddivari Y, Nettleship JE, Owens RJ, Antson AA, Waterman DG. S-Adenosyl-S-carboxymethyl-L-homocysteine: a novel cofactor found in the putative tRNA-modifying enzyme CmoA. Acta Crystallogr D Biol Crystallogr. 2013 Jun;69(Pt 6):1090-8. doi: 10.1107/S0907444913004939. Epub 2013 May 15.

Aricescu AR, Owens RJ. Expression of recombinant glycoproteins in mammalian cells: towards an integrative approach to structural biology. Curr Opin Struct Biol. 2013 Jun;23(3):345-56. doi: 10.1016/ Epub 2013 Apr 25. Review. PMID: 23623336


Nettleship JE. Structural Biology of Glycoproteins, Glycosylation, Dr. Stefana Petrescu (Ed.). ISBN: 978-953-51-0771-2. 2012.

Lobley CM, Aller P, Douangamath A, Reddivari Y, Bumann M, Bird LE, Nettleship JE, Brandao-Neto J, Owens RJ, O'Toole PW, Walsh MA. Structure of ribose 5-phosphate isomerase from the probiotic bacterium Lactobacillus salivarius UCC118. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Dec 1;68(Pt 12):1427-33.

Yates LA, Lumb CN, Brahme NN, Zalyte R, Bird LE, De Colibus L, Owens RJ, Calderwood DA, Sansom MS, Gilbert RJ. Structural and functional characterization of the kindlin-1 pleckstrin homology domain. J Biol Chem. 2012 Dec 21;287(52):43246-61.

Porta C, Xu X, Loureiro S, Paramasivam S, Ren J, Al-Khalil T, Burman A, Jackson T, Belsham GJ, Curry S, Lomonossoff GP, Parida S, Paton D, Li Y, Wilsden G, Ferris N, Owens R, Kotecha A, Fry E, Stuart DI, Charleston B, Jones IM. Efficient production of foot-and-mouth disease virus empty capsids in insect cells following down regulation of 3C protease activity. J Virol Methods. 2012 Nov 19.

Berry JL, Phelan MM, Collins RF, Adomavicius T, Tønjum T, Frye SA, Bird L, Owens R, Ford RC, Lian LY, Derrick JP. Structure and assembly of a trans-periplasmic channel for type IV pili in Neisseria meningitidis. PLoS Pathog. 2012 Sep;8(9):e1002923.

Nettleship JE, Flanagan A, Rahman-Huq N, Hamer R, Owens RJ. Converting monoclonal antibodies into Fab fragments for transient expression in mammalian cells. Methods Mol Biol. 2012;801:137-59.

Owen RL, Axford D, Nettleship JE, Owens RJ, Robinson JI, Morgan AW, DorJ AS,Lebon G, Tate CG, Fry EE, Ren J, Stuart DI, Evans G. Outrunning free radicals in room-temperature macromolecular crystallography. Acta Crystallogr D Biol Crystallogr. 2012 Jul;68(Pt 7):810-8.

Sainsbury S, Ren J, Saunders NJ, Stuart DI, Owens RJ. Structure of theregulatory domain of the LysR family regulator NMB2055 (MetR-like protein) from Neisseria meningitidis. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jul 1;68(Pt 7):730-7.

Axford D, Owen RL, Aishima J, Foadi J, Morgan AW, Robinson JI, Nettleship JE,Owens RJ, Moraes I, Fry EE, Grimes JM, Harlos K, Kotecha A, Ren J, Sutton G,Walter TS, Stuart DI, Evans G. In situ macromolecular crystallography using microbeams.Acta Crystallogr D Biol Crystallogr. 2012 May;68(Pt 5):592-600.


Wilke S, Groebe L, Maffenbeier V, Jäger V, Gossen M, Josewski J, Duda A, Polle L, Owens RJ, Wirth D, Heinz DW, van den Heuvel J, Büssow K. Streamlining homogeneous glycoprotein production for biophysical and structural applications by targeted cell line development. PLoS One. 2011;6(12):e27829. Epub 2011 Dec 9.

Green VL, Verma A, Owens RJ, Phillips SE, Carr SB. Structure of New Delhi metallo-β-lactamase 1 (NDM-1). Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Oct 1;67(Pt 10):1160-4.

Bird LE. High throughput construction and small scale expression screening of multi-tag vectors in Escherichia coli. Methods. 2011 Sep;55(1):29-37.

Daniel E, Lin B, Diprose JM, Griffiths SL, Morris C, Berry IM, Owens RJ, Blake R, Wilson KS, Stuart DI, Esnouf RM. xtalPiMS: a PiMS-based web application for the management and monitoring of crystallization trials. J Struct Biol. 2011 Aug;175(2):230-5.

Holdom MD, Davies AM, Nettleship JE, Bagby SC, Dhaliwal B, Girardi E, Hunt J, Gould HJ, Beavil AJ, McDonnell JM, Owens RJ, Sutton BJ. Conformational changes in IgE contribute to its uniquely slow dissociation rate from receptor FcεRI. Nat Struct Mol Biol. 2011 May;18(5):571-6.

Busso D, Peleg Y, Heidebrecht T, Romier C, Jacobovitch Y, Dantes A, Salim L, Troesch E, Schuetz A, Heinemann U, Folkers GE, Geerlof A, Wilmanns M, Polewacz A, Quedenau C, Büssow K, Adamson R, Blagova E, Walton J, Cartwright JL, Bird LE, Owens RJ, Berrow NS, Wilson KS, Sussman JL, Perrakis A, Celie PH. Expression of protein complexes using multiple Escherichia coli protein co-expression systems: A benchmarking study. J Struct Biol. 2011 Aug;175(2):159-70.

Renella R, Roberts NA, Brown JM, De Gobbi M, Bird LE, Hassanali T, Sharpe JA, Sloane-Stanley J, Ferguson DJ, Cordell J, Buckle VJ, Higgs DR, Wood WG. Codanin-1 mutations in congenital dyserythropoietic anemia type 1 affect HP1Α localization in erythroblasts. Blood. 2011 Mar 1. [Epub ahead of print]

Nettleship JE. Chapter 22: Hydrophilic Interaction Liquid Chromatography In The Characterisation Of Glycoproteins (in Hydrophilic Interaction Liquid Chromatography (HILIC) and Advanced Applications eds. Wang PG, He W.), 2011, CRC Press, 523-550.

Sainsbury S, Bird L, Rao V, Shepherd SM, Stuart DI, Hunter WN, Owens RJ, Ren J. Crystal structures of penicillin-binding protein 3 from Pseudomonas aeruginosa: comparison of native and antibiotic-bound forms. J Mol Biol. 2011 Jan 7;405(1):173-84.

Owens RJ, Nettleship JE. (Eds.) Functional and Structural Proteomics of Glycoproteins, 2011, Springer.


Bird LE, Ren J, Nettleship JE, Folkers GE, Owens RJ, Stammers DK. Novel structural features in two ZHX homeodomains derived from a systematic study ofsingle and multiple domains. BMC Struct Biol. 2010 May 28;10(1):13.

Ren J, Sainsbury S, Nettleship JE, Saunders NJ, Owens RJ. The crystal structure of NGO0477 from Neisseria gonorrhoeae reveals a novel protein fold incorporating a helix-turn-helix motif. Proteins. 2010 May 15;78(7):1798-802.

Liu C, Walter TS, Huang P, Zhang S, Zhu X, Wu Y, Wedderburn LR, Tang P, Owens RJ, Stuart DI, Ren J, Gao B. Structural and Functional Insights of RANKL-RANK Interaction and Signaling. J Immunol. 2010 Jun 15;184(12):6910-9.

Sainsbury S, Ren J, Nettleship JE, Saunders NJ, Stuart DI, Owens RJ. The structure of a reduced form of OxyR from Neisseria meningitidis. BMC Struct Biol.2010 May 17;10(1):10.

Hitchman RB, Possee RD, Siaterli E, Richards KS, Clayton AJ, Bird LE, Owens RJ, Carpentier DE, King FL, Danquah JO, Spink KG, King LA. Improved expression of secreted and membrane-targeted proteins in insect cells. Biotechnol Appl Biochem. 2010 Jun 21;56(3):85-93.

Assenberg R, Delmas O, Ren J, Vidalain PO, Verma A, Larrous F, Graham SC, Tangy F, Grimes JM, Bourhy H. Structure of the nucleoprotein binding domain of Mokola virus phosphoprotein. J Virol. 2010 Jan;84(2):1089-96.

Assenberg R, Delmas O, Morin B, Graham SC, De Lamballerie X, Laubert C, Coutard B, Grimes JM, Neyts J, Owens RJ, Brandt BW, Gorbalenya A, Tucker P, Stuart DI, Canard B, Bourhy H. Genomics and structure/function studies of Rhabdoviridae proteins involved in replication and transcription. Antiviral Res. 2010 Aug;87(2):149-61.

Nettleship JE, Assenberg R, Diprose JM, Rahman-Huq N, Owens RJ. Recent advances in the production of proteins in insect and mammalian cells for structural biology. J Struct Biol. Oct;172(1):55-65.


Bollati M, Alvarez K, Assenberg R, Baronti C, Canard B, Cook S, Coutard B,Decroly E, De Lambellerie X, Gould EA, Grard G, Grimes JM, Hilgenfeld R, Jansson AM, Malet H, Mancini EJ, Mastrangelo E, Mattevi A, Milani M, Moureau G, Neyts J, Owens RJ, Ren J, Selisko B, Speroni S, Steuber H, Stuart DI, Unge T, Bolognesi M. Structure and functionality in flavivirus NS-proteins: Perspectives for drug design. Antiviral Res. 2009 Nov 27. [Epub ahead of print]

Bowden TA, Aricescu AR, Nettleship JE, Siebold C, Rahman-Huq N, Owens RJ, Stuart DI, Jones EY. Structural plasticity of eph receptor A4 facilitates cross-class ephrin signaling. Structure. 2009 Oct 14;17(10):1386-97.

van Boxel GI, Stewart-Jones G, Holmes S, Sainsbury S, Shepherd D, Gillespie GM, Harlos K, Stuart DI, Owens R, Jones EY. Some lessons from the systematic production and structural analysis of soluble alphabeta T-cell receptors. J Immunol Methods. 2009 Oct 31;350(1-2):14-21.

Assenberg R, Mastrangelo E, Walter TS, Verma A, Milani M, Owens RJ, Stuart DI,Grimes JM, Mancini EJ. Crystal structure of a novel conformational state of theFlavivirus NS3 protein: Implications for Polyprotein Processing and Viral Replication. J. Virol. 2009 Dec;83(24):12895-906.

Hitchman RB, Possee RD, Crombie AT, Chambers A, Ho K, Siaterli E, Lissina O,Sternard H, Novy R, Loomis K, Bird LE, Owens RJ, King LA. Genetic modification of a baculovirus vector for increased expression in insect cells. Cell Biol Toxicol. 2009 Aug 5. [Epub ahead of print]

Clayton A, Siebold C, Gilbert RJ, Sutton GC, Harlos K, McIlhinney RA, Jones EY, Aricescu AR. Crystal Structure of the GluR2 Amino-Terminal Domain Provides Insights into the Architecture and Assembly of Ionotropic Glutamate Receptors. J Mol Biol. 2009 Oct 9;392(5):1125-32.

Bishop B, Aricescu AR, Harlos K, O'Callaghan CA, Jones EY, Siebold C. Structural insights into hedgehog ligand sequestration by the human hedgehog-interacting protein HHIP. Nat Struct Mol Biol. 2009 Jul;16(7):698-703. Epub 2009 Jun 28

Hatherley D, Graham SC, Harlos K, Stuart DI, Barclay AN. Structure of signal regulatory protein {alpha}: A link to antigen receptor evolution. J Biol Chem. 2009 Jul 23. [Epub ahead of print]

Paesen GC, Siebold C, Dallas ML, Peers C, Harlos K, Nuttall PA, Nunn MA, Stuart DI, Esnouf RM. An ion-channel modulator from the saliva of the brown eartick has a highly modified Kunitz/BPTI structure. J Mol Biol. 2009 Jun 19;389(4):734-47. Epub 2009 Apr 24.

Crispin M, Bowden TA, Coles CH, Harlos K, Aricescu AR, Harvey DJ, Stuart DI, Jones EY. Carbohydrate and domain architecture of an immature antibody glycoform exhibiting enhanced effector functions. J Mol Biol. 2009 Apr 17;387(5):1061-6

Harkiolaki M, Holmes SL, Svendsen P, Gregersen JW, Jensen LT, McMahon R,Friese MA, van Boxel G, Etzensperger R, Tzartos JS, Kranc K, Sainsbury S, Harlos K, Mellins ED, Palace J, Esiri MM, van der Merwe PA, Jones EY, Fugger L. T cell-mediated autoimmune disease due to low-affinity crossreactivity to common microbial peptides. Immunity. 2009 Mar 20;30(3):348-57.

Harkiolaki M, Tsirka T, Lewitzky M, Simister PC, Joshi D, Bird LE, Jones EY, O'Reilly N, Feller SM. Distinct binding modes of two epitopes in Gab2 that interact with the SH3C domain of Grb2. Structure. 2009 Jun 10;17(6):809-22.

Walter TS, Liu C, Huang P, Zhang S, Wedderburn LR, Gao B, Owens RJ, Stuart DI,Tang P, Ren J. Crystallization and preliminary X-ray analysis of mouse RANK and its complex with RANKL. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Jun 1;65(Pt 6):597-600.

Sainsbury S, Lane LA, Ren J, Gilbert RJ, Saunders NJ, Robinson CV, Stuart DI, Owens RJ. The structure of CrgA from Neisseria meningitidis reveals a new octameric assembly state for LysR transcriptional regulators. Nucleic Acids Res. 2009 Aug;37(14):4545-58.

Nichols CE, Sainsbury S, Ren J, Walter TS, Verma A, Stammers DK, Saunders NJ, Owens RJ. The structure of NMB1585, a MarR-family regulator from Neisseria meningitidis. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Mar 1;65(Pt 3):204-9. Epub 2009 Feb 26.

Nettleship JE, Rahman-Huq N, Owens RJ. The production of glycoproteins by transient expression in Mammalian cells.Methods Mol Biol. 2009;498:245-63.

Berrow NS, Alderton D, Owens RJ. The Precise Engineering of Expression Vectors Using High-Throughput In-Fusiontrade mark PCR Cloning.Methods Mol Biol. 2009;498:75-90.


Graham SC, Assenberg R, Delmas O, Verma A, Gholami A, Talbi C, Owens RJ, Stuart DI, Grimes JM, Bourhy H. Rhabdovirus matrix protein structures reveal a novel mode of self-association.PLoS Pathog. 2008 Dec;4(12):e1000251. Epub 2008 Dec 26.

Possee RD, Hitchman RB, Richards KS, Mann SG, Siaterli E, Nixon CP, Irving H, Assenberg R, Alderton D, Owens RJ, King LA. Generation of baculovirus vectors for the high-throughput production of proteins in insect cells. Biotechnol Bioeng. 2008 Dec 15;101(6):1115-22.

Abrescia NG, Grimes JM, Kivelä HM, Assenberg R, Sutton GC, Butcher SJ, Bamford JK, Bamford DH, Stuart DI. Insights into virus evolution and membrane biogenesis from the structure of the marine lipid-containing bacteriophage PM2. Mol Cell. 2008 Sep 5;31(5):749-61.

Sainsbury S, Ren J, Saunders NJ, Stuart DI, Owens RJ. Crystallization and preliminary X-ray analysis of CrgA, a LysR-type transcriptional regulator from pathogenic Neisseria meningitidis MC58. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Sep 1;64(Pt 9):797-801.

Graham SC, Bahar MW, Cooray S, Chen RA, Whalen DM, Abrescia NG, Alderton D, Owens RJ, Stuart DI, Smith GL, Grimes JM. Vaccinia virus proteins A52 and B14 Share a Bcl-2-like fold but have evolved to inhibit NF-kappaB rather than apoptosis. PLoS Pathog. 2008 Aug 15;4(8):e1000128

Nettleship JE, Ren J, Rahman N, Berrow NS, Hatherley D, Barclay AN, Owens RJ. A pipeline for the production of antibody fragments for structural studies using transient expression in HEK 293T cells. Protein Expr Purif. 2008 Jul 10. [Epub ahead of print]

Kotaka M, Johnson C, Lamb HK, Hawkins AR, Ren J, Stammers DK.Structural analysis of the recognition of the negative regulator NmrA and DNA by the zinc finger from the GATA-type transcription factor AreA. J Mol Biol. 2008 Aug 29;381(2):373-82.

Meier C, Carter LG, Sainsbury S, Mancini EJ, Owens RJ, Stuart DI, Esnouf RM The Crystal Structure of UMP Kinase from Bacillus anthracis (BA1797) Reveals an Allosteric Nucleotide-Binding Site. J Mol Biol. 2008 Jul 3. [Epub ahead of print]

Bahar MW, Kenyon JC, Putz MM, Abrescia NG, Pease JE, Wise EL, Stuart DI, SmithGL, Grimes JM.Structure and function of A41, a vaccinia virus chemokine binding protein. PLoS Pathog. 2008 Jan;4(1):e5.

Hatherley D, Graham SC, Turner J, Harlos K, Stuart DI, Barclay AN.Paired Receptor Specificity Explained by Structures of Signal Regulatory Proteins Alone and Complexed with CD47. Mol Cell. 2008 Jul 25;31(2):266-77.

Bowden TA, Aricescu AR, Gilbert RJ, Grimes JM, Jones EY, Stuart DI.Structural basis of Nipah and Hendra virus attachment to their cell-surfacereceptor ephrin-B2.Nat Struct Mol Biol. 2008 Jun;15(6):567-72.

Ren J, Nettleship JE, Sainsbury S, Saunders NJ, Owens RJ.Structure of the cold-shock domain protein from Neisseria meningitidis reveals a strand-exchanged dimer.Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Apr 1;64(Pt 4):247-51.

Nettleship JE, Brown J, Groves MR and Geerlof, A Methods for protein characterization by mass spectrometry, thermal shift (ThermoFluor) assay, and multi-angle or static light scattering. (in Methods in Molecular Biology: Structural proteomics: High throughput methods eds Guss, M, Kobe, B and Huber, T.) Vol 426. 2008 Humana Press.

Assenberg R, Delmas O, Graham SC, Verma A, Berrow N, Stuart DI, Owens RJ,Bourhy H, Grimes JM. Expression, purification and crystallization of a lyssavirus matrix (M) protein.Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Apr 1;64(Pt 4):258-62.

Au K, Ren J, Walter TS, Harlos K, Nettleship JE, Owens RJ, Stuart DI, Esnouf RM.Structures of an alanine racemase from Bacillus anthracis (BA0252) in thepresence and absence of (R)-1-aminoethylphosphonic acid (L-Ala-P).Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 May 1;64(Pt 5):327-33.

Coutard B, Gorbalenya AE, Snijder EJ, Leontovich AM, Poupon A, De Lamballerie X, Charrel R, Gould EA, Gunther S, Norder H, Klempa B, Bourhy H, Rohayem J, L'hermite E, Nordlund P, Stuart DI, Owens RJ, Grimes JM, Tucker PA, Bolognesi M, Mattevi A, Coll M, Jones TA, Aqvist J, Unge T, Hilgenfeld R, Bricogne G, Neyts J, La Colla P, Puerstinger G, Gonzalez JP, Leroy E, Cambillau C, Romette JL, Canard B. The VIZIER project: Preparedness against pathogenic RNA viruses. Antiviral Res. 2008 Apr;78(1):37-46.

Structural Genomics Consortium; China Structural Genomics Consortium; Northeast Structural Genomics Consortium, Gr�slund S, Nordlund P, Weigelt J, Bray J, Gileadi O, Knapp S, Oppermann U, Arrowsmith C, Hui R, Ming J, dhe-Paganon S, Park HW, Savchenko A, Yee A, Edwards A, Vincentelli R, Cambillau C, Kim R, Kim SH, Rao Z, Shi Y, Terwilliger TC, Kim CY, Hung LW, Waldo GS, Peleg Y, Albeck S, Unger T, Dym O, Prilusky J, Sussman JL, Stevens RC, Lesley SA, Wilson IA, Joachimiak A, Collart F, Dementieva I, Donnelly MI, Eschenfeldt WH, Kim Y, Stols L, Wu R, Zhou M, Burley SK, Emtage JS, Sauder JM, Thompson D, Bain K, Luz J, Gheyi T, Zhang F, Atwell S, Almo SC, Bonanno JB, Fiser A, Swaminathan S, Studier FW, Chance MR, Sali A, Acton TB, Xiao R, Zhao L, Ma LC, Hunt JF, Tong L, Cunningham K, Inouye M, Anderson S, Janjua H, Shastry R, Ho CK, Wang D, Wang H, Jiang M, Montelione GT, Stuart DI, Owens RJ, Daenke S, Sch�tz A, Heinemann U, Yokoyama S, B�ssow K, Gunsalus KC. Protein production and purification. Nat Methods. 2008 Feb;5(2):135-46. Review.

Zaccai NR, Carter LG, Berrow NS, Sainsbury S, Nettleship JE, Walter TS, Harlos, K, Owens RJ, Wilson KS, Stuart DI, Esnouf RM. Crystal structure of a 3-oxoacyl-(acylcarrier protein) reductase (BA3989) from Bacillus anthracis at 2.4-A resolution.Proteins. 2008 Feb 1;70(2):562-7.

Walter TS, Mancini EJ, Kadlec J, Graham SC, Assenberg R, Ren J, Sainsbury S, Owens RJ, Stuart DI, Grimes JM, Harlos K.Semi-automated microseeding of nanolitre crystallization experiments. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Jan 1;64(Pt1):14-8.


Mancini EJ, Assenberg R, Verma A, Walter TS, Tuma R, Grimes JM, Owens RJ, Stuart DI.Structure of the Murray Valley encephalitis virus RNA helicase at 1.9 Angstrom resolution. Protein Sci. 2007 Oct;16(10):2294-300.

Graham SC, Bahar MW, Abrescia NG, Smith GL, Stuart DI, Grimes JM. Structure of CrmE, a virus-encoded tumour necrosis factor receptor. J Mol Biol. 2007 Sep 21;372(3):660-71.

Meier C, Carter LG, Winter G, Owens RJ, Stuart DI, Esnouf RM. Structure of 5-formyltetrahydrofolate cyclo-ligase from Bacillus anthracis(BA4489).Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Mar 1;63(Pt 3):168-72.

Cooray S, Bahar MW, Abrescia NG, McVey CE, Bartlett NW, Chen RA, Stuart DI, Grimes JM, Smith GL. Functional and structural studies of the vaccinia virus virulence factor N1 reveal a Bcl-2-like anti-apoptotic protein. J Gen Virol. 2007 Jun;88(Pt 6):1656-66.

Paesen GC, Siebold C, Harlos K, Peacey MF, Nuttall PA, Stuart DI. A tick protein with a modified Kunitz fold inhibits human tryptase. J Mol Biol. 2007 May 11;368(4):1172-86.

Hatherley D, Harlos K, Dunlop DC, Stuart DI, Barclay AN. The structure of the macrophage signal regulatory protein alpha (SIRPalpha) inhibitory receptor reveals a binding face reminiscent of that used by T cell receptors. J Biol Chem. 2007 May 11;282(19):14567-75.

Ren J, Sainsbury S, Combs SE, Capper RG, Jordan PW, Berrow NS, Stammers DK, Saunders NJ, Owens RJ. The structure and transcriptional analysis of a global regulator from Neisseria meningitidis. J Biol Chem. 2007 May 11;282(19):14655-64.

Chotiyarnwong P, Stewart-Jones GB, Tarry MJ, Dejnirattisai W, Siebold C, Koch M, Stuart DI, Harlos K, Malasit P, Screaton G, Mongkolsapaya J, Jones EY. Humidity control as a strategy for lattice optimization applied to crystals of HLA-A*1101 complexed with variant peptides from dengue virus. Acta Crystallograph Sect F Struct Biol Cryst Commun. 2007 May 1;63(Pt 5):386-92.

Berrow NS, Alderton D, Sainsbury S, Nettleship J, Assenberg R, Rahman N, Stuart DI, Owens RJ. A versatile ligation-independent cloning method suitable for high-throughput expression screening applications. Nucleic Acids Res. Feb 22; 2007;35(6):e45. Epub 2007

Chang VT, Crispin M, Aricescu AR, Harvey DJ, Nettleship JE, Fennelly JA, Yu C, Boles KS, Evans EJ, Stuart DI, Dwek RA, Jones EY, Owens RJ, Davis SJ. Glycoprotein structural genomics: solving the glycosylation problem. Structure. 2007 Mar;15(3):267-73.

Meier C, Carter LG, Winter G, Owens RJ, Stuart DI, Esnouf RM. Structure of 5-formyltetrahydrofolate cyclo-ligase from Bacillus anthracis (BA4489). Acta Crystallograph Sect F Struct Biol Cryst Commun. 2007 Mar 1;63(Pt 3):168-72. Epub 2007 Feb 23.

Nettleship JE, Aplin R, Radu Aricescu A, Evans EJ, Davis SJ, Crispin M, Owens RJ. Analysis of variable N-glycosylation site occupancy in glycoproteins by liquid chromatography electrospray ionization mass spectrometry. Anal Biochem. 2007 Feb 1;361(1):149-51.


Alzari, P.M., H. Berglund, N. S.Berrow, E. Blagova, D. Busso, C. Cambillau, V. Campanacci, E. Christodoulou, S. Eiler, M. Fogg, G. Folkers, A. Geerlof, D. Hart, A. Haouz, M. D. Herman, S. Macieira, P. Nordlund, A. Perrakis, S. Quevillon-Cheruel, F. Tarendeau, H. van Tilbeurgh, T. Unger, M. P. A. Luna-Vargas, M. Velarde, M.Wilmanns and R. J.Owens. Implementation of semi-automated cloning and prokaryotic expression screening: the impact of SPINE Acta Cryst. (2006). D62, 1103-1113.

Albeck, S., P. Alzari, C. Andreini, L. Banci, I.M. Berry, I. Bertini, C. Cambillau, B. Canard, L. Carter, S. Cohen, J.M. Diprose, O. Dym, R.M. Esnouf, C. Felder, F. Ferron, F. Guillemot, R. Hamer, M. Jelloul, R.A. Laskowski, S. Longhi, R. Lopez, C. Luchinat, H. Malet, T. Mochel, R.J. Morris, L. Moulinier, T. Oinn, A. Pajon, Y. Peleg, A. Perrakis, O. Poch, J. Prilusky, A. Rachedi, R. Ripp, A. Rosato, I. Silman, D.I. Stuart, J.L. Sussman, J.-C. Thierry, J.D. Thompson, J.M. Thornton, T. Unger, B. Vaughan, W. Vranken, J.D. Watson, G. Whamond and Kim Henrick. SPINE bioinformatics and data management aspects of high-throughput structural biology Acta Cryst. (2006). D62, 1184-1195 .

Aricescu, A.R., R. Assenberg, R.M. Bill, D. Busso, V.C.Chang, S.J. Davis, A. Dubrovsky, L. Gustafsson, K. Hedfalk, U. Heinemann, I.M. Jones, D. Ksiazek, C. Lang, K. Maskos, A. Messerschmidt, S. Macieira, Y. Peleg, A. Perrakis, A. Poterszman, G. Schneider, T.K. Sixma, J.L. Sussman, G. Sutton, N. Tarboureich, T. Zeev-Ben-Mordehai and E.Y. Jones.Eukaryotic expression: Developments for Structural Proteomics. Acta Cryst. (2006). D62, 1114-1124.

Au K, Berrow NS, Blagova E, Boucher IW, Boyle MP, Brannigan JA, Carter LG, Dierks T, Folkers G, Grenha R, Harlos K, Kaptein R, Kalliomaa AK, Levdikov VM, Meier C, Milioti N, Moroz O, Muller A, Owens RJ, Rzechorzek N, Sainsbury S, Stuart DI, Walter TS, Waterman DG, Wilkinson AJ, Wilson KS, Zaccai N, Esnouf RM, Fogg MJ.Application of high-throughput technologies to a structural-proteomics type analysis of B. anthracis. Acta Cryst. (2006). D62, 1267-1275

Banci, L., I. Bertini, S. Cusack, R.N. de Jong, U. Heinemann, E.Y. Jones, F. Kozielski, K. Maskos, A. Messerschmidt, R. Owens, A. Perrakis, A. Poterszman, G. Schneider, C. Siebold, I. Silman, T. Sixma, G. Stewart-Jones, J.L. Sussman, J-C. Thierry, and Dino Moras. First steps towards effective methods in exploiting high throughput technologies for the determination of human protein structures of high biomedical value. Acta Cryst. (2006). D62, 1208-1217

Berrow NS K. Büssow, B. Coutard, J. Diprose, M. Ekberg, G.E. Folkers, N. Levy, V. Lieu, R.J. Owens, Y. Peleg, C. Pinaglia, S. Quevillon-Cheruel, L. Salim, C. Scheich, R. Vincentelli and Didier Busso.Recombinant protein expression and solubility screening in Escherichia coli: a comparative study. Acta Cryst. (2006). D62, 1218-1226.

Berry, I.M., O. Dym, R.M. Esnouf, K.Harlos, R. Meged, A. Perrakis, J. L. Sussman, T. S. Walter, J. Wilson and Albrecht Messerschmidt. SPINE high throughput crystallization, crystal imaging and recognition techniques: current state, performance analysis, new technologies and future aspects. Acta Cryst. (2006). D62, 1137-1149.

Dhaliwal B, Ren J, Lockyer M, Charles I, Hawkins AR, Stammers DK. Structure of Staphylococcus aureus cytidine monophosphate kinase in complexwith cytidine 5'-monophosphate. Acta Crystallograph Sect F Struct Biol Cryst Commun. 2006 Aug 1;62(Pt 8):710-5.

Esnouf RM, Love CA, Harlos K, Stuart DI, Jones EY. Structure determination of human semaphorin 4D as an example of the use of MAD in non-optimal cases. Acta Crystallogr D Biol Crystallogr. 2006 Jan;62(Pt 1):108-15

Esnouf, R.M., R. Hamer, J.L. Sussman, I. Silman, D. Trudgian, Z-R.Yang, and Jaime Prilusky Honing the in silico toolkit for detecting protein disorder. Acta Cryst. (2006). D62, 1260-1266

Fogg, M.J., P. Alzari, M. Bahar, I. Bertini, J-M. Betton, W.P. Burmeister, C. Cambillau, B. Canard, M. Carrondo, M. Coll, S. Daenke, O. Dym, M-P. Egloff, F. J. Enguita, A. Geerlof, A. Haouz, T. A. Jones, Qingjun Ma, S. N. Manica, M. Migliardi, P. Nordlund, R. Owens, Y. Peleg, , G. Schneider, R. Schnell, D. I. Stuart, N. Tarbouriech, T. Unge, A. J. Wilkinson, M. Wilmanns, K. S. Wilson, O. Zimhony and Jonathan M. Grimes. Application of the use of high throughput technologies to the determination of protein structures of bacterial and viral pathogens. Acta Cryst. (2006). D62, 1196-1207

Gadola SD, M. Koch, J. Marles-Wright, N.M. Lissin, D. Shepherd, G. Matulis, K. Harlos, P.M. Villiger, D.I. Stuart, B.K. Jakobsen, V. Cerundolo and E.Y. Jones. (2006) "Structure analysis and binding kinetics of three human CD1d/αGalactosylceramide specific TCRs using different Variable α chains paired to Variable β11." J. Exp. Med. 203, 699-710

Geerlof, A, J. Brown, B. Coutard, M-P. Egloff, F. J. Enguita, M. J. Fogg, R.J.C. Gilbert, M.R. Groves, A. Haouz, J.E. Nettleship, P. Nordlund, R.J. Owens, M. Ruff, S. Sainsbury, D.I. Svergun and Matthias Wilmanns. The Impact of Protein Characterisation in Structural Proteomics. Acta Cryst. (2006). D62, 1125-1136

Kotaka M, Ren J, Lockyer M, Hawkins AR, Stammers DK. Structures of R- and T-state Escherichia coli aspartokinase III: Mechanisms of the allosteric transition and inhibition by lysine. J Biol Chem. 2006 Oct 20;281(42):31544-52.

Kotaka M, Dhaliwal B, Ren J, Nichols CE, Angell R, Lockyer M, Hawkins AR, Stammers DK. Structures of S. aureus thymidylate kinase reveal an atypical active siteconfiguration and an intermediate conformational state upon substrate binding. Protein Sci. 2006 Apr;15(4):774-84.

Meier C, Aricescu AR, Assenberg R, Aplin RT, Gilbert RG, Grimes JG, Stuart DI (2006) Crystal Structure of Orf9b, a lipid-binding protein from SARS Coronavirus Structure 14(7): 1157-1165.

Nichols CE, Sainsbury S, Berrow, NS, Alderton D, Saunders NJ, Stammers, DK, Owens RJ. Structure of the PII signal transduction protein of Neisseria meningitidis at 1.85 Å resolution. Acta Crystallograph Sect F Struct. Genomics Commun. 2006 June ;62 (Pt 6):494-497

Nichols CE, Johnson C, Lockyer M, Charles IG, Lamb HK, Hawkins AR, StammersDK. Structural characterization of Salmonella typhimurium YeaZ, an M22O-sialoglycoprotein endopeptidase homolog. Proteins. 2006 Jul 1;64(1):111-23.

El Omari K, Ren J, Bird LE, Bona MK, Klarmann G, LeGrice SF, Stammers DK. Molecular architecture and ligand recognition determinants for T4 RNA ligase. J Biol Chem. 2006 Jan 20;281(3):1573-9.

El Omari K, Bronckaers A, Liekens S, Perez-Perez MJ, Balzarini J, StammersDK. Structural basis for non-competitive product inhibition in human thymidinephosphorylase: implications for drug design. Biochem J. 2006 Oct 15;399(2):199-204.

El Omari K, Dhaliwal, B., Lockyer M, Charles I, Hawkins AR, Stammers DK Structure of Staphylococcus aureus guanylate monophosphate kinase (2006) Acta Crystallograph Sect F Struct Biol Cryst Commun 2006 Oct 1;62(Pt 10):949-53.

El Omari K, Solaroli N, Karlsson A, Balzarini J, Stammers DK. Structure of vaccinia virus thymidine kinase in complex with dTTP: insights for drug design. BMC Struct Biol. 2006 Oct 24;6:22.

Owens RJ, Nettleship, JE, Berrow, NS, Sainsbury S, Aricescu RA, Stuart DI and Stammers DK High throughput cloning, expression and purification in Macromolecular Crystallography: conventional and high throughput methods-A Practical Approach (Eds. M.Sanderson & J.Skelly) OUP (in press).

Walter TS, Meier C, Assenberg R, Au K, Ren J, Verma A, Nettleship JE, Owens RJ, Stuart DI, Grimes J Lysine methylation as routine rescue strategy for crystallization Structure WAYS & MEANS 2006 Nov;14(11):1617-22.


Abrescia NG, Kivela HM, Grimes JM, Bamford JK, Bamford DH, Stuart DI. Preliminary crystallographic analysis of the major capsid protein P2 of the lipid-containing bacteriophage PM2. Acta Crystallogr. F Struct Biol Cryst Commun. 2005 Aug 1;61(Pt 8):762-5

Aricescu AR, W-C. Hon, C. Siebold, W. Lu, P.A. van der Merwe and E.Y. Jones. (2006) "Molecular analysis of receptor protein tyrosine phosphatase µ mediated cell adhesion." EMBO J. 25 701-712

Berry I, Wilson J, Diprose J, Stuart D, Fuller S, Esnouf R. Image storage for automated crystallization imaging systems.Int J Neural Syst. 2005 Dec;15(6):415

J-L. Chen, G. Stewart-Jones, G. Bossi, N.M. Lissin, L. Wooldridge, E.M.L. Choi, G. Held, P.R. Dunbar, R.M. Esnouf, M. Sami, J.M. Boulter, P. Rizkallah, C. Renner, A. Sewell, P.A. van der Merwe, B.K. Jakobsen, G. Griffiths, E.Y. Jones and V. Cerundolo. (2005) "Structural and kinetic basis for heightened immunogenicity of T-cell vaccines." J. Exp. Med. 201 1243-1255.

Evans EJ, R.M. Esnouf, R. Manso-Sancho, R.J. Gilbert, J.R. James, C. Yu, C. Vowles, T.Hanke, B. Walse, T. Hünig, P. Sørensen, D.I. Stuart and S.J. Davis (2005) Crystal structure of a soluble CD28-Fab complex. Nat Immunol.;6(3):271-9.-25.

Gilbert RJ, Beales L, Blond D, Simon MN, Lin BY, Chisari FV, Stuart DI, Rowlands DJ. Hepatitis B small surface antigen particles are octahedral. Proc Natl Acad Sci U S A. 2005 Oct 11;102(41):14783-8.

Koch M, Stronge VS, Shepherd D, Gadola SD, Mathew B, Ritter G, Fersht AR, Besra GS, Schmidt RR, Jones EY, Cerundolo V. (2005) The crystal structure of human CD1d with and without alpha-galactosylceramide. Nat Immunol.6(8):819-26.

Laurila MR, Salgado PS, Makeyev EV, Nettelship J, Stuart DI, Grimes JM, Bamford DH. (2005)Gene silencing pathway RNA-dependent RNA polymerase of Neurospora crassa:yeast expression and crystallization of selenomethionated QDE-1 protein.J Struct Biol. 149(1):111-5.

Mayo CJ, Diprose JM, Walter TS, Berry IM, Wilson J, Owens RJ, Jones EY,Harlos K, Stuart DI, Esnouf RM. (2005) Benefits of automated crystallization plate tracking, imaging, and analysis. Structure (Camb).13(2):175-82.

Nettleship JE, Walter TS, Aplin R, Stammers DK, Owens RJ. (2005) Sample preparation and mass-spectrometric characterization of crystal-derived protein samples. Acta Crystallogr D Biol Crystallogr. 61(Pt 5):643-5.

Newman J, Egan D, Walter TS, Meged R, Berry I, Ben Jelloul M, Sussman JL,Stuart DI, Perrakis A. (2005) Towards rationalization of crystallization screening for small- to medium-sizedacademic laboratories: the PACT/JCSG+ strategy.Acta Crystallogr D Biol Crystallogr. 2005 Oct;61(Pt 10):1426-31

Meier C, Mancini EJ, Bamford DH, Walsh MA, Stuart DI, Grimes JM. Overcoming the false-minima problem in direct methods: structure determination of the packaging enzyme P4 from bacteriophage phi13. Acta Crystallogr D Biol Crystallogr. 2005 61:1238-44

El Omari K, Bird LE, Nichols CE, Ren J, Stammers DK.(2005) Crystal structure of CC3 (TIP30): implications for its role as a tumor suppressor. J Biol Chem.280(18):18229-36.

Pajon A, Ionides J, Diprose J, Fillon J, Fogh R, Ashton AW, Berman H,Boucher W, Cygler M, Deleury E, Esnouf R, Janin J, Kim R, Krimm I, Lawson CL,Oeuillet E, Poupon A, Raymond S, Stevens T, van Tilbeurgh H, Westbrook J, Wood P,Ulrich E, Vranken W, Xueli L, Laue E, Stuart DI, Henrick K.(2005) Design of a data model for developing laboratory information management and analysis systems for protein production. Proteins. 1;58(2):278-84

Ren J, Sainsbury S, Berrow NS, Alderton D, Nettleship JE, Stammers DK,Saunders NJ, Owens RJ.(2005) Crystal structure of nitrogen regulatory protein IIANtr from Neisseria meningitidis. BMC Struct Biol. 10;5:13.

P. Roche, J. Brown, A. Denley, B.E. Forbes, J.C. Wallace, E.Y. Jones and R.M. Esnouf (2005) A computational model for the IGF-II/IGF2R complex that is predictive of mutational and surface plasmon resonance data. PROTEINS 64 758-768

Siebold C , N S. Berrow, T S. Walter, K Harlos, R J. Owens, D I. Stuart, J R. Terman, A L. Kolodkin, R. Jeroen Pasterkamp E. Yvonne Jones (2005). High-resolution structure of the catalytic region of MICAL (molecule interacting with CasL), a multi-domain flavoenzyme-signaling molecule Proc Natl Acad Sci U S A. 2005 Nov 15;102(46):16836-16841.

Stewart-Jones G, G. Bossi, N.M. Lissin, L. Wooldridge, E.M.L. Choi, G. Held, P.R. Dunbar, R.M. Esnouf, M. Sami, J.M. Boulter, P. Rizkallah, C. Renner, A. Sewell, P.A. van der Merwe, B.K. Jakobsen, G. Griffiths, E.Y. Jones and V. Cerundolo. (2005) "Structural and kinetic basis for heightened immunogenicity of T-cell vaccines." J. Exp. Med. 201 1243-1255

Walter TS, Diprose JM, Mayo CJ, Siebold C, Pickford MG, Carter L, Sutton GC,Berrow NS, Brown J, Berry IM, Stewart-Jones GB, Grimes JM, Stammers DK, EsnoufRM, Jones EY, Owens RJ, Stuart DI, Harlos K. A procedure for setting up high-throughput nanolitre crystallization experiments. Crystallization workflow for initial screening, automated storage, imaging and optimization. (2005) Acta Crystallogr D Biol Crystallogr. Jun;61(Pt 6):651-7.

Yang ZR, Thomson R, McNeil P, Esnouf RM.( 2005) "RONN: the bio-basis function neural network technique applied to the detection of natively disordered regions in proteins. Bioinformatics. 15;21(16):3369-76.


Batuwangala T, Shepherd D, Gadola SD, Gibson KJC, Zaccai NR, Besra GS, Cerundolo V and Jones EY (2004). "The crystal structure of human CD1b with a bound bacterial glycolipid" J. Immunol. 172, 2382-2388.

Batuwangala T, Leduc M, Gibbins JM, Bon C and Jones EY (2004). "The crystal structure of the snake venom toxin convulxin" Acta Crystallogr. D60, 46-53.

Dhaliwal, D, C.E. Nichols, J. Ren, M. Lockyer, I. Charles, A.R. Hawkins and D.K. Stammers (2004) "Crystallographic Studies of Shikimate Binding and Induced Conformational Changes in Mycobacterium tuberculosis Shikimate Kinase" FEBS Lett. 2004 10;574(1-3):49-54.

Lee, S., V. Knott, J. Jovanovic, K. Harlos, J.G. Grimes, C. Choulier, H.J. Mardon, D.I. Stuart and P.A. Handford (2004) "Structure Of The Integrin-Binding Fragment From Fibrillin-1 Gives New Insights Into Microfibril Organisation" Structure 12, 1-20.

Nichols, C.E., M. Lockyer, A.R. Hawkins and D. K. Stammers (2004) "Crystal Structures of Staphylococcus aureus type I Dehydroquinase from enzyme turnover experiments" Proteins: Structure, Function and Bioinformatics 56, 625-628.

Nichols, C.E., A.R. Hawkins and D. K. Stammers (2004) "Structure of the "open" form of Aspergillus nidulans dehydroquinate synthase at 1.7Å resolution from crystals grown following enzyme turnover" Acta Cryst. D60, 971-973.

Nichols, C.E. B. Dhaliwal, M. Lockyer, A.R. Hawkins and D. K. Stammers (2004) "High resolution structures reveal details of domain closure and "half-of-sites-reactivity" in Escherichia coli aspartate β-semialdehyde dehydrogenase" Mol Biol. 2004 13;341(3):797-806.

Nichols, C.E., J. Ren, B. Dhaliwal, M. Lockyer, I.G. Charles, A.R. Hawkins and D.K. Stammers (2004) "Comparison of ligand induced conformational changes and domain closure mechanisms between prokaryotic and eukaryotic dehydroquinate synthases" J Mol Biol. 2004 22;343(3):533-46.

Ren, J., C.E. Nichols, P.P. Chamberlain, K.L. Weaver, S.A. Short and D.K. Stammers (2004) "Crystal structures of HIV-1 reverse transcriptases mutated at codons 100, 106 & 108 and mechanisms of resistance to non-nucleoside inhibitors" J. Mol. Biol. 336, 569-578.

Siebold, C., B.E. Hansen, J.R. Wyer, K. Harlos, R.E. Esnouf, A. Svejgaard, J.I. Bell, J.L. Strominger, E.Y. Jones and L. Fugger (2004) "Crystal structure of HLA-DQ0602 that protects against type 1 diabetes and confers strong susceptibility to narcolepsy." Proc. Natl. Acad. Sci. USA 101, 1999-2004.

G. Sutton, E. Fry, L. Carter, S. Sainsbury, T. Walter , J. Nettleship , N. Berrow , R. Owens , R. Gilbert, A. Davidson, S. Siddell, L.M. Poon, J. Diprose , D. Alderton , M. Walsh , J. M. Grimes and D. I. Stuart (2004) "The nsp9 Replicase Protein of SARS-Coronavirus, Structure and Functional Insights" Structure 12, 341-353.


Bird, L.E., P.P. Chamberlain, J. Ren, G.B. Stewart-Jones, D.I. Stuart and D.K. Stammers (2003) "Expression purification and crystallisation of HIV-2 reverse transcriptase" Prot. Exp. and Purif. 27, 12-18.

Bird, L.E., J. Ren, A. Wright, K.D. Leslie, B. Degrève, J. Balzarini and D.K. Stammers (2003) "Crystal structure of varicella zoster virus thymidine kinase" J.Biol.Chem. 278, 24680-24687.

Brown, J., T.S. Walter, L. Carter, N. Abrescia, R. Aricescu, T.D. Batuwangala, L.E. Bird, N. Brown, P. Chamberlain, S. Davis, E. Dubinina, J. Endicott, J. Fennelly, R. Gilbert, M. Harkiolaki, W-C. Hon, F. Kimberley, C.A. Love, E. Mancini, R. Manso-Sancho, C. Nichols, R. Robinson, G. Sutton, N. Schueller, M. Sleeman, G. Stewart-Jones, M. Vuong, J. Welburn, Z. Zhang, D.K. Stammers, R. Owens, E.Y. Jones, K. Harlos and D.I. Stuart (2003) "A procedure for setting up high-throughput nanolitre crystallization experiments II: Crystallization results" J. Appl. Cryst. 36, 315-318.

Lamb, H.K., K. Leslie, A.L. Dodds, M. Nutley, A. Cooper, C. Johnson, P. Thompson, D.K. Stammers and A.R. Hawkins (2003) "The negative transcriptional regulator NmrA discriminates between oxidized and reduced dinucleotides" J.Biol.Chem. 278, 32107-32114.

Love, C.A., K. Harlos, N. Mavaddat, S.J. Davis, D.I. Stuart, E.Y. Jones and R.M. Esnouf (2003) "The ligand-binding face of the semaphorins revealed by the high resolution crystal structure of SEMA4D." Nature Struct. Biol. 10, 843-848.

Nichols, C.E., J. Ren, H. Lamb, A.R. Hawkins and D.K. Stammers (2003) "Ligand induced conformational changes in Aspergillus nidulans dehydroquinate synthase and a mechanism for domain closure" J. Mol. Biol. 327, 129-144.

Walter, T. S., Diprose, J., Brown, J., Pickford, M., Stuart, D. I. & Harlos, K. (2003) J. Appl. Cryst. A procedure for setting up high-throughput, nanolitre crystallization experiments. I Protocol design and validation. J. Appl. Cryst. 36, 308-314