Crystallization Facilities at the OPPF-UK

Xll imagers

Equipment

The OPPF provides access to a high-throughput crystallization pipeline. Crystallization experiments are carried out in 96-well Greiner plates using nanolitre-scale droplets (typically 100 nL of protein + 100 nL of reagents). Crystallization plates are stored and imaged at regular intervals at either 4°C or 21°C in automated storage systems. Images are accessible to the users via a web-interface.

Instruments available include:

  • Hydra instrument to transfer crystallisation solution from blocks to Greiner plates
  • Cartesian Microsys instrument to dispense nanolitre droplets
  • Two Formulatrix Storage and Imaging System capable of storing 1000 plates at 21°C
  • A Formulatrix Storage and Imaging System capable of storing 1000 plates at 4°C
  • Two stereo-microscopes with Pixera imaging cameras to view crystallization plates manually

Publications

  • Brown J, Walter TS, Carter L, Abrescia N, Aricescu R, Batuwangala TD, Bird LE, Brown N, Chamberlain P, Davis S, Dubinina E, Endicott J, Fennelly J, Gilbert R, Harkiolaki M, Hon W-C, Kimberley F, Love CA, Mancini E, Manso-Sancho R, Nichols C, Robinson R, Sutton G, Schueller N, Sleeman M, Stewart-Jones G, Vuong M, Welburn J, Zhang Z, Stammers DK, Owens R, Jones EY, Harlos K and Stuart DI (2003). "A procedure for setting up high-throughput nanolitre crystallization experiments II: Crystallization results" J. Appl. Cryst. 36, 315-318. PubMed
  • Walter TS, Diprose JM, Mayo CJ, Siebold C, Pickford MG, Carter L, Sutton GC, Berrow NS, Brown J, Berry IM, Stewart-Jones GB, Grimes JM, Stammers DK, Esnouf RM, Jones EY, Owens RJ, Stuart DI and Harlos K (2005). "A procedure for setting up high-throughput nanolitre crystallization experiments. Crystallization workflow for initial screening, automated storage, imaging and optimization" Acta Crystallogr., D61, 651-657. PubMed